Uric Acid

938

Substrates & Metabolites

Uric acid

Serum or Heparinised-plasma

R1: 4 x 42 mL;
R2: 4 x 11 mL,
Ready for use

Instructions for Use  |  Safety Data Sheet

For professional use only.

Uric acid is the principle product of the catabolism of purine, adenosine and guanosine nucleotides. Approximately 400 mg of uric acid are synthesised daily; food intake accounts for a further 300 mg. About 75% of the uric acid excreted is eliminated in the urine. Most of uric acid is secreted in the gastrointestinal tract where it is degraded by bacterial enzymes into allantoin and other compounds. The amount of uric acid present is determined essentially by the life style adopted, e.g. alimentary habits, alcohol consumption, physical activity, assumption of pharmaceutical products may all influence hyperuricemia. Some the most dangerous complications of uricemia include acute and chronic attacks of gout, and pathological conditions related to renal function.

Uric acid is converted by uricase and hydrogen peroxide which, under the catalytic influence of peroxide (POD), oxidises compound, reacts with 4aminophenazone and 3,5diclorophenol-sulphonate giving a red coloured compound, whose colour intensity is directly proportional to the uric acid concentration in the tested sample.
Uric Acid + 2H2O + O2 --(Uricase)--> Allantoin + CO2 + 2H2O2
3,5-diclorophenolsulphonate + 2H2O2 + 4-Aminophenazone --(POD)--> Compound dyed

Store at 2 - 8°C; protect from direct light. When correctly stored, the reagents are stable up the expiry date reported on the label. A slight variation in the composition of the reagent may occur between batches, but this has no effect on the test results. After opening, the vial R1 and R2 are stable 30 days if recapped immediately and protected from contamination, evaporation, direct light and stored at correct temperature.

Multicalibrator (R0300000006)
Normal control serum (R0400000006)
Pathological control serum (R0400000106)