Glucose

1369

Substrates & Metabolites

Glucose

Serum or EDTA-plasma

Reagent: 6 x 50 mL,
Ready for use

Instructions for Use  |  Safety Data Sheet

For professional use only.

Glucose is the result of the chemical decomposition of carbohydrates introduced in the diet and is the primary source of energy for the human organism. When the energetic intake is greater than the energy used, the glucose in excess is converted into fat and glycogen which will be deposited in the form of energy reserves, in the adipose tissue, in the liver and muscles. The glucose concentration in human blood is regulated, within a narrow range, by the action of regulatory hormones such as insulin, glucagon or epinephrine. The most widespread disorder of carbohydrate metabolism is diabetes mellitus, which is evidenced by high blood glucose levels.

In accordance with Trinder’s reaction, glucose oxidase (GOD) oxidates glucose to gluconic acid with the formation of hydrogen peroxide, which in the presence of peroxidase (POD), 4-aminophenazone and phenol gives rise to a coloured compound, the intensity of which is directly proportional to the glucose concentration in the sample.
Glucose + O2 --(GOD)--> Gluconic Acid + H2O2
2H2O2 + 4-Aminophenazone + Phenol --(POD)--> CouloredCompoud

Store at 2 - 8°C and protect from direct light. When correctly stored, the reagents are stable up the expiry date reported on the label. A slight variation in the composition of the reagent may occur between batches, but this has no effect on the test results. After opening, the vial R1 and R2 are stable 30 days if recapped immediately and protected from contamination, evaporation, direct light and stored at correct temperature.

Multicalibrator (R0300000006)
Normal control serum (R0400000006)
Pathological control serum (R0400000106)