HbA1C
Quantitative determination of Hemoglobin A1c (HbA1c) in human blood by turbidimetric immunoassay. The determination of HbA1c is most commonly performed for the evaluation of glycemic control in diabetes mellitus. HbA1c values provide an indication of glucose levels over the preceding 4-8 weeks. A higher HbA1c value indicates poorer glycemic control.
Tests per Kit
100
Test Category
Specific Proteins
Target Analytes
Glycated haemoglobin (HbA1c)
Sample Type
Fresh EDTA blood
Kit Components
Upon request
Tech Docs
Instructions for Use | Safety Data Sheet
Intended Use
For professional in vitro diagnostic use only.
Clinical Significance
Throughout the circulatory life of the red cell, HbA1c is formed continuously by the adduction of glucose to the N-terminal of the haemoglobin beta chain. The process, which is non-enzymatic, reflects the average exposure of haemoglobin to glucose over an extended period. In a classical study, Trivelli et al. showed HbA1c in diabetic subjects to be elevated 2-3 folds over the levels found in normal individuals. Several investigators have recommended that HbA1c serves as an indicator of metabolic control of the diabetic, since HbA1c levels approach normal values for diabetics in metabolic control. HbA1c has been defined operationally as the “fast fraction” haemoglobins (HbA1a, A1b, A1c) that elute first during column chromatography with cation-exchange resins. The nonglycosylated haemoglobin, which consists of the bulk of the haemoglobin has been designated HbA0.
Test Principle
This method utilises the interaction of antigen and antibody to determine the HbA1c in whole EDTA blood. HbA1c in test samples is absorbed onto the surface of latex particles, which react with Anti-HbA1c (antigen-antibody reaction) and gives agglutination. The amount of agglutination is measured as absorbance. The HbA1c value is obtained from a calibration curve.
Stability & Storage
The reagents are stable until expiry date when kept at 2-8°C. After opening the reagents are stable for 1 month at 2-8°C. Do not freeze.
Required but Not Supplied
Hemoglobin A1c Standard Set (R0300000003)
Hemoglobin A1c Control Low (R0400000007)
Hemoglobin A1c Control High (R0400000004)
Hemolysing Solution for HbA1c, 500 mL (R3140000042)
