Triglycerides

200

Lipids

Triglycerides

Serum or plasma samples

Reagent: 3 x 14 mL,
Ready to use

Instructions for Use  |  Safety Data Sheet

For professional use only.

Triglycerides constitute 95% of the lipids reserve in the human organism and the predominant form of glycerol esters present in the plasma. Thanks to the action of lipases and biliary salts, triglycerides are hydrolysed into glycerol and fatty acids, and transported in the plasma by the apolipoproteins. The lipoproteins with the highest percentage of triglycerides are the chilomicrons and the very low--density lipoproteins (VLDL). The combination of an increase in LDL Cholesterol and triglycerides is an aggravating factor in the risk of coronary disease. Hypertriglyceridemia is a common disease in adults and is associated with diseases such as diabetes mellitus, insulinresistance or obesity. Secondary hypertriglyceridemia on the other hand is associated with much more serious conditions such as liver and renal disease, hyperthyroidism and pancreatitis.

Glycerol released by the hydrolysis of triglycerides with lipoprotein-lipase (LPL), is transformed by glycerol-chinase (GK) to glycerol-3-phosphate which in turn is oxidased by glycerol-phosphate-oxidase (GPO) to di-hydroxyacetone-phosphate with the formation of hydrogen peroxide; this, in turn, in the presence of peroxidase (POD), reacts with ethyl-sulfopropyl-toluidine (ESPT) and 4-aminophenazone, giving rise to a coloured compound, the intensity of which is directly proportional to the triglyceride concentration in the sample.
Triglycerides --(LPL)--> Glycerol + Fatty Acids
Glycerol + ATP --(GK)--> Glycerol-3-phospate + ADP
Glycerol-3-phospate + O2 --(GPO)--> Di-hidroxyacetate-phospate + H2O2
2H2O2 + 4-aminophenazone + ESPT --(POD)--> Colouredcompound

Store at 2 - 8°C and protect from direct light. When correctly stored, the reagents are stable up to the expiry date reported on the label. A slight variation in the composition of the reagents may occur between batches, but this has no effect on the test results. After opening, the vials are stable 30 days if recapped immediately and protected from contamination, evaporation, direct light and stored at correct temperature.

Multicalibrator (R0300000006)
Normal control serum (R0400000006)
Pathological control serum (R0400000106)