Total Protein
Product for use in the quantitative determination in vitro of the concentration of Total Proteins in human serum or plasma. The results of the test must always be interpreted in conjunction with the clinical context.
Tests per Kit
200
Test Category
General Proteins
Target Analytes
Total protein
Sample Type
Serum or plasma
Kit Components
Reagent: 3 x 14 mL,
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Instructions for Use | Safety Data Sheet
Intended Use
For professional use only.
Clinical Significance
Proteinemia is a test useful to quantify the proteins present in the blood. Often, this parameter is measured as part of the panel of analyses performed during routine check-ups, so it is frequently used in assessing the patient's general health status. The concentration of proteins in the blood can provide general information about nutritional status, useful, in particular, when the patient has inexplicably lost weight. Proteinemia can be prescribed together with other tests to understand the cause of an abnormal accumulation of fluid in the tissues (oedema) and as a support to the diagnosis of certain liver and kidney diseases.
Test Principle
The determination of Total Proteins can be performed both with physical and chemical methods. Its determination is based on the chemical method of biureto-tartrate. Copper ions in alkaline medium react with substances containing two or more peptide groups to form a violet complex, this reaction is referred to as the biuret reaction (1). The intensity of the colour is directly proportional to the concentration of proteins in the sample.
Stability & Storage
Store at 15 - 25°C and protect from direct light. When correctly stored, the reagents are stable up the expiry date reported on the label. A slight variation in the composition of the reagent may occur between batches, but this has no effect on the test results. After opening, the vials are stable 30 days if recapped immediately and protected from contamination, evaporation, direct light and stored at correct temperature
Required but Not Supplied
Multicalibrator (R0300000006)
Normal control serum (R0400000006)
Pathological control serum (R0400000106)

















