Phosphorus
Product for use in the quantitative determination in vitro of the concentration of Inorganic phosphorus in human serum or urine. The results of the test must always be interpreted in conjunction with the clinical picture.
Tests per Kit
200
Test Category
Minerals & Trace Elements
Target Analytes
Inorganic phosphorus
Sample Type
Fresh, haemolysis-free serum
Kit Components
Reagent: 3 x 14 mL,
Ready to useTech Docs
Instructions for Use | Safety Data Sheet
Intended Use
For professional use only.
Clinical Significance
Phosphorus is an important element present in the human body in the form of inorganic or organic phosphate. In serum exists in the form of both monovalent and divalent phosphate anions. About 10 % of the phosphate present in serum is bound to proteins, 35 % is found in combination with sodium, calcium and magnesium and the remaining percentage is free. Phosphate is one of the main components of hydroxyapatite present in bones, plays an important role as a high-energy phosphate bond and is a vital component for the activity of numerous enzyme systems. Hypophosphatemia is a relatively common condition in the nosocomial population, detectable in several causes such as: stimulation of insulin secretion that promotes the transport of phosphates into cells and respiratory alkalosis. A loss of phosphates by the kidney can be the cause of a low level of phosphates in serum. Hyperphosphatemia is usually related to renal failure. A decrease in glomerular filtration limits renal phosphate excretion resulting in the development of hyperphosphatemia.
Test Principle
The method measures the absorbance at 340 nm of unreduced phosphomolybdate and allows the determination of inorganic phosphates in serum. Inorganic phosphorus reacts, in the presence of sulfuric acid, with molybdate ammonium to give an unreduced complex that can be read at 340 nm.
Inorganic phosphorus + H2SO4 + ammonium molybdate → PhosphomolybdateStability & Storage
Store at 2 - 8°C and protect from direct light. When correctly stored, the reagents are stable up the expiry date reported on the label. A slight variation in the composition of the reagent may occur between batches, but this has no effect on the test results. After opening, the vial are stable 30 days if recapped immediately and protected from contamination, evaporation, direct light and stored at correct temperature.
Required but Not Supplied
Multicalibrator (R0300000006)
Normal control serum (R0400000006)
Pathological control serum (R0400000106)
