Human haemoglobin (FOB)

100

Specific Proteins

Human haemoglobin (faecal)

Human stool

R1: 1 x 18.3 mL;
R2: 1 x 2.5 mL,
Ready to use

Instructions for Use  |  Safety Data Sheet

For professional in vitro diagnostic use only.

Colorectal cancer is the second leading cause of illness and death in Western world. The screening with faecal occult blood tests is based on the concept that important target colonic neoplasm, such as early-stage cancer and large adenomatous polyps. Colorectal cancer is also associated with local acute inflammatory reaction being visualised, in some cases, by white cell neutrophil scanning. Haemoglobin is the iron-containing oxygen-transport protein in the red blood cells of all vertebrates that may be leaked into gastrointestinal tract and then discharged with the faeces in gastrointestinal bleeding diseases. When gastrointestinal blood is lost, the stool will contain a combination of intact or nearly intact haemoglobin, intact heme and heme-derived porphyrins in amounts that depend on the site and amount of bleeding and the transit time through the gut. FOB tests detect intact or nearly intact human haemoglobin, being a very specific technique for detecting loss of blood from the lower intestine. Consult the Universal Stool Extraction Vial instructions for use for correct stool sample extraction.

FOB turbidimetric assay is based on agglutination reactions. These involve in vitro aggregation of microscopic latex particles. This aggregation consists in the specific reaction between antigen and antibodies, antigen contained in the sample and the antibodies anti-antigen coated on polystyrene latex particles. The sample is mixed with a suspension containing antibodies against the antigen bound to latex particles. If antigen is present in the sample it will react with the antibodies and form an aggregate. If no antigen is present in the sample the mixture will keep its appearance as a smooth suspension. Such turbidity is measured as an increase in absorbance at the determinate wave and is proportional to the quantity of antigen contained in the sample.

The reagents are stable until expiry date on the label when stored at 2-8°C. DO NOT FREEZE After opening the reagents are stable for 1 month at 2-8°C.

Universal Stool Extraction Tubes (R3330000061)
Standard Set FOB (R3330000064)
Control Set FOB (R3330000068)