Creatinine

200

Substrates & Metabolites

Creatinine

Serum or urine

R1: 3 x 12.6 mL;
R2: 1 x 4.4 mL,
Ready to use

Instructions for Use  |  Safety Data Sheet

For professional use only.

Creatinine is synthesised in the kidneys, the liver and the pancreas, and is subsequently transported in the blood to other organs such as the muscles and the brain. As the production of endogenous creatinine is proportional to the muscular mass, it varies according to age and sex. The influence of the amount of meat taken in daily in the diet can be considered to be approximately 10%. In general, however, daily fluctuations in the creatinine present in the diet cause only minor variations in the daily excretion levels. High creatinine levels are seen in cases of acute or chronic renal insufficiency and in dehydration.

Jaffé (1) was the first to describe the reaction which takes place between creatinine and an alkaline picrate solution, with formation of a coloured compound. Subsequently the method used for the determination of serum creatinine was modified to improve its specificity. Creatinine reacts with alkaline picrate, forming a coloured compound which is measured at 505 nm. The rate of the colour formation is proportional to the creatinine concentration.

Store at 2 - 8°C; protect from direct light. When correctly stored, the reagents are stable up to the expiry date reported on the label. A slight variation in the composition of the reagents may occur between batches, but this has no effect on the test results. After opening, the vial R1 and R2 are stable 30 days if recapped immediately and protected from contamination, evaporation, direct light and stored at correct temperature

Multicalibrator (R0300000006)
Normal control serum (R0400000006)
Pathological control serum (R0400000106)