Cholesterol PAP
Product for use in the quantitative determination in vitro of the concentration of the Cholesterol in human serum or plasma. The results of the test must always be interpreted in conjunction with the clinical context.
Tests per Kit
200
Test Category
Lipids
Target Analytes
Total cholesterol
Sample Type
Serum or Heparinised-plasma
Kit Components
Reagent: 3 x 14 mL,
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Instructions for Use | Safety Data Sheet
Intended Use
For professional use only.
Clinical Significance
Cholesterol is an essential component of the cell membrane of all animal cells. Cholesterol forms the basis for the synthesis of steroid hormones such as aldosterone, cortisone and testosterone; it is also essential for development of the embryo. Although part of the cholesterol present in the human body is derived from the diet, it is mostly produced in the liver and used largely for bile production. As cholesterol, like all lipids, is insoluble in the blood, aggregated complexes such as lipoproteins are used for its transportation in circulation. For this reason, when in medicine the term “cholesterol” is used, reference is in fact being made to lipoproteins which circulate in the blood, the concentration of which is known as cholesterolemia.
Test Principle
Esterified cholesterol is hydrolysed into free cholesterol and fatty acid by cholesterol esterase (CE). Cholesterol oxidase (CO) then oxidates the free cholesterol to cholest-4en-3-one with formation of hydrogen peroxide which, in the presence of peroxidase (POD), reacts with hydroxybenzoate (HBA and 4-aminophenazone, giving rise to a coloured compound, the intensity of which is read at a wavelength of 510 nm; the result is directly proportional to the cholesterol concentration in the sample.
Cholesterol Esters --(CE)--> Cholesterol + Fatty Acids
Cholesterol + O2 --(CO)--> Cholest-4-en-3-one + H2O2
2H2O2 + HBA + 4-Aminophenazone --(POD)--> Coloured CompoundStorage Conditions
Store at 2 - 8°C and protect from direct light. When correctly stored, the reagents are stable up the expiry date reported on the label. A slight variation in the composition of the reagent may occur between batches, but this has no effect on the test results. After opening, the vial R1 and R2 are stable 30 days if recapped immediately and protected from contamination, evaporation, direct light and stored at correct temperature.
Required but Not Supplied
Multicalibrator (R0300000006)
Normal control serum (R0400000006)
Pathological control serum (R0400000106)
