Calprotectin

100

Specific Proteins

Human calprotectin (faecal)

Human stool

R1: 1 x 18.3 mL;
R2: 1 x 2.7 mL,
Ready to use

Instructions for Use  |  Safety Data Sheet

For professional in vitro diagnostic use only.

Calprotectin is a neutrophil cytosolic protein with antimicrobial properties, which is present at increased concentration in stool samples during bowel inflammation. Calprotectin is released by activation of leukocytes, giving increased levels in plasma, cerebral spinal fluid, synovial fluid, urine or stools as a consequence of disease in the relevant organ(s). Calprotectin inhibits zincdependent enzyme systems, as a result kills microbes and induces apoptosis in normal and cancer cells. In the presence of calcium, calprotectin is remarkably resistant to protelolytic degradation and so is stable in stools kept at room temperature for 7 days.

Calprotectin latex turbidimetric assay is based on agglutination reactions. These involve in vitro aggregation of microscopic latex particles. This aggregation consists in the specific reaction between antigen and antibodies, antigen contained in the sample and the antibodies anti-antigen coated on polystyrene latex particles. The sample is mixed with a suspension containing antibodies against the antigen bound to latex particles. If antigen is present in the sample, it will react with the antibodies and form an aggregate. If no antigen is present in the sample the mixture will keep its appearance as a smooth suspension. Such turbidity is measured as an increase in absorbance at the determinate wave and is proportional to the quantity of antigen contained in the sample.

The reagents are stable until expiry date on the label when stored at 2-8°C. DO NOT FREEZE After opening the reagents are stable for 1 month at 2-8°C.

Universal Stool Extraction Tubes (R3330000061)
Standard Set Calprotectin (R3330000065)
Control Set Calprotectin (R3330000069)