BCG Albumin

200

General Proteins

Albumin

Serum or plasma

Reagent: 3 x 14 mL,
Ready to use

Instructions for Use  |  Safety Data Sheet

For professional use only.

Albumin is the plasma protein which is present in the largest quantities, constituting about half the total amount of proteins. It is synthesised in the hepatic parenchymal cells. The rate of synthesis is regulated primarily by COP (colloid osmotic pressure) and secondly, by the supply of protein. The normal half-life of albumin in plasma is 15-19 days. Increased albumin levels are present only in cases of acute dehydration. Decreased albumin levels are seen in numerous clinical conditions such as: analbuminemia, hepatopathic inflammation, loss in the urine, oedema and ascites. Rodkey in 1965 (1) and Hernandez in 1967 (2) described methods for the determination of albumin using Bromocresol Green (BCG). In 1972 Doumas (3) proposed a modified BCG method, establishing a reaction pH of 3.8. The method is based on Doumas’s study, with a further modification to improve the linearity and stability of the final reaction colour.

In a suitable buffer solution, serum or plasma albumin binds to Bromocresol Green (BCG). The intensity of the colour which develops is directly proportional to the amount of albumin present in the sample. The presence of a tensioactive agent in the reagent increases the linearity of the reaction.

Store at 15-25°C; protect from direct light. When correctly stored, the reagent is stable up to the expiry date reported on the label. A slight variation in the composition of the components may occur between batches, but this has no effect on the test results. After opening, the vial R1 and R2 are stable 30 days if recapped immediately and protected from contamination, evaporation, direct light and stored at correct temperature.

Multicalibrator (R0300000006)
Normal control serum (R0400000006)
Pathological control serum (R0400000106)