AST / GOT

200

Enzymes

Aspartate aminotransferase (AST / GOT)

Fresh non-haemolysed serum or heparinised plasma

R1: 3 x 12.6 mL;
R2: 1 x 4 mL,
Ready to use

Instructions for Use  |  Safety Data Sheet

For professional use only.

Aspartate aminotransferase (AST/GOT) and alanine aminotransferase (ALT/GPT) belong to the group of aminotransferases or transaminases; these catalyse the reversible transformation of α-cheto-acids into aminoacids through the transfer of amino groups. AST and ALT are present in human plasma, bile, cerebrospinal fluid and saliva. The serum ALT and AST levels are increased in viral hepatitis and in other forms of hepatic disease. In spite of the fact that, in such cases, both enzymes show raised serum levels, ALT is the more specific enzyme for the diagnosis of hepatic damage. AST levels can rise in connection with cardiac or skeletal muscle damage, in addition to hepatic parenchymal tissue damage.

In the presence of 2-oxoglutarate, aspartate is transformed into oxalacetate and glutamate by the aspartate aminotransferase (AST/GPT) present in the sample. In the presence of NADH and malate-dehydrogenase (MDH), oxalacetate is transformed into malate and NAD. The consumption of NADH over a given period of time, determined at λ 340 nm, is proportional to the GOT concentration in the test sample.
L-Aspartate + 2-Oxglutarate --(AST)--> L-Glutamate + Oxalacetate
Oxalacetate + NADH + H+ --(MDH)--> D-Malate + NAD+

Store at 2 - 8°C and protect from direct light. When correctly stored, the reagent is stable up to the expiry date reported on the label. A slight variation in the composition of the components may occur between batches, but this has no effect on the test results. After opening, the vial R1 and R2 are stable 30 days if recapped immediately and protected from contamination, evaporation, direct light and stored at correct temperature

Multicalibrator (R0300000006)
Normal control serum (R0400000006)
Pathological control serum (R0400000106)