ALT GPT

200

Enzymes

Alanine aminotransferase (ALT / GPT)

Fresh non-haemolysed serum or heparinised plasma

R1: 3 x 12.6 mL;
R2: 1 x 4 mL,
Ready to use

Instructions for Use  |  Safety Data Sheet

For professional use only.

Alanine aminotransferase (ALT/GPT) and aspartate aminotransferase (AST/GOT) are part of the group of aminotransferases or transaminases. These catalyse the reversible transformation of α-cheto-acids into aminoacids through the transfer of amino groups. AST and ALT are present in human plasma, bile, cerebrospinal fluid and saliva. In viral hepatitis and other forms of hepatic disease, the serum ALT level increases even before the appearance of clinical signs and pathological symptoms. The ALT activity can reach values 100-times higher than the upper reference range limit, although in most cases the increase corresponds to 20-25 times the normal values.

In the presence of 2-oxoglutarate, alanine is transformed into pyruvate and glutamate by the GPT present in the sample. In the presence of NADH and Lactate-Dhydrogenase (LDH), Pyruvate is transformed into lactate and NAD. The consumption of NADH over a given period of time, determined at λ 340 nm, is proportional to the GPT concentration in the test sample.

Store at 2 - 8 °C away from direct light. When stored as described above, reagents are stable until the expiry date stated on the label. A slight variation in the composition of the reagents can occur from batch to batch, without affecting the test results. After opening, they are stable for 30 days if closed immediately and protected from contamination, evaporation, direct light and stored at the correct temperature.

Multicalibrator (R0300000006)
Normal control serum (R0400000006)
Pathological control serum (R0400000106)